Dr. Guillermo Federico Ehrenbolger

Dr. Guillermo Federico ­ Ehrenbolger [✓]

Piktogramm der Rolle 'IT-Support Department'
IT-Support Department
(IT Support Supervisor)

Contact

Address

Room: F211
Hauptstraße 47
69117 Heidelberg

PhD Fellow Researcher

Universidad Nacional de Bueno Aires (UBA), Facultad de Ciencias Exacta y Naturales (FCEyN), Instituto Nacional de Tecnología Agropecuaria (INTA) with a scholarship awarded by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

Full-time position, period: Apr 2010 - Apr 2015

Link to the PhD Thesis: http://hdl.handle.net/20.500.12110/tesis_n6711_Ehrenbolger


Description:

My doctoral research focused on the initial transcriptional defence responses of sunflower against Sclerotinia head rot (SHR), caused by the fungus Sclerotinia sclerotiorum. In order to evaluate the level of resistance in various inbred lines, I used phenotypic, genotypic, and molecular analyses.

The Purity of the materials was ensured through SSR molecular markers, and the resistance levels were evaluated using incidence, severity, intensity, and the area under the disease-progress curve (AUDPC). To analyze the changes in gene expression, I used microarrays and identified response genes based on factors such as fold changes, p-values, co-expressed genes, and multivariate analysis. To achieve this I employed various tools, including Infostat, MapMan, R + WGCNA library, Blast2Go, MeV, and Galaxy.

The identified cluster of differentially expressed genes were specific to the susceptible or resistant lines, indicating that the responses were independent. Their functional annotation identified various functions, including proteolysis, membrane receptors and Ca2+ signaling, cellular redox, and plant hormones auxins and ethylene

Morover, my research identified 43 candidate resistance genes (and 47 putative susceptible genes) with crucial roles in plant defense, such as: oxidative stress detection, transcription factors, ubiquitination, peptidases inhibitors, membrane zinc transporter proteins, synthesis of amino acids, and dynamics of the cytoskeleton. I also designed two techniques to detect the early presence of S. sclerotiorum, prior to the onset of the first SHR symptoms appear. One approach relied on optical observations while the other on PCR.

My work has deepened our understanding of the complex molecular mechanisms involved in the triggering and evolution of S. sclerotiorum infectious process in sunflower as well as to the identification of genes involved in different stages of SHR disease, and has the potential to enhance the improvement of this oleaginous crop.

Please refer to the lecture index (LSF) for past courses..

Biology science communicator in english - IB and IGCSE programmes.Biology science communicator in english - IB and IGCSE programmes.

Tarbut School (https://www.tarbut.edu.ar/)
Mar 2020 - Feb 2022
Buenos Aires, Argentina

During my time at this prestigious educational institution, I created engaging educational content and facilitated student learning for International Baccalaureate (IB) and Cambridge IGCSE programmes in biology. Designed lesson plans, assignments, and assessments, delivered up-to-date lectures, and provided individual support to students, parents and school members. Furthermore, I assessed student learning outcomes and collaborated with other educators to develop effective and innovative teaching strategies. My main goal was to inspire students to develop a passion for knowledge and equipped them with tools and skills for future success.


Biology, Science and Pysichal-chemistry communicator in english - IB programme.Biology, Science and Pysichal-chemistry communicator in english - IB programme.

St. John's School (https://www.stjohns.edu.ar/)
Feb 2015 - Dec 2017
Beccar, Buenos Aires, Argentina

In the course of my work, I performed as a dynamic communicator for the International Baccalaureate (IB) programme for the subjects Biology, Science, and Physical-Chemistry. I was able to create captivating educational content, deliver engaging lectures and activities that captivated students' attention. I also provided comprehensive support to students, including one-on-one assistance and group discussions that fostered learning and comprehension. My objectives involved assessing student progress, collaborating with peers to develop effective teaching strategies, and, whenever possible, inspiring students to develop a love for science and knowledge as a whole. Through my work, I provided a stong foundation for students to achieve personal and academic growth.


Biology teacher in english - IGCSE ProgrammeBiology teacher in english - IGCSE Programme

Holy Cross School (https://holycross.ar/)
Feb 2014 - Mar 2015
Victoria, Buenos Aires, Argentina

As a biology science communicator for the Cambridge IGCSE Programme, I had the honor of teaching at the renowned Holy Cross, where I inspired students to excel in their personal academic pursuits. The main objective through my dynamic instruction and innovative lesson plans, was to cultivate a deep understanding of Biology among my students, convincing them that knowledge is a tool that can be applied on their daily lives. I worked collaboratively with my colleagues to create an exceptional educational experience for our students, and constantly assessed their progress to provide constructive feedback. My time at Holy Cross was an enriching experience that allowed me to grow as a communicator thanks to the amazing team members and students.

Latest curriculum vitae can be found in my Linkedin profile
Link: https://www.linkedin.com/in/ehrenbolger/

Doctoral thesis: 

Identification and functional characterization of candidate resistance genes of sunflower against the pathogen Sclerotinia sclerotiorum using a transcriptomic approach.
Link: https://bibliotecadigital.exactas.uba.ar/download/tesis/tesis_n6711_Ehrenbolger.pdf

Scientific publications:

RNA-seq analysis reveals sunflower responses to Sclerotinia Head Rot at early stages of infection. 
Science Reports. Jan 1, 2020.  
Link: https://www.nature.com/articles/s41598-020-70315-4

Functional Genomics and Transgenesis Applied to Sunflower Breeding Functional Genomics and Transgenesis Applied to Sunflower Breeding Sunflowers: Growth and Development, environmental influences and pests/diseases. 
Nova Science Publishers, Inc. ISBN: 978-1-63117-347-9. Jan 1, 2014
Link: https://www.novapublishers.com/wp-content/uploads/2019/04/978-1-63117-347-9_ch6.pdf



Personal site: https://valkanoise.github.io/

Github site: https://github.com/valkanoise/

Linkedin site: https://www.linkedin.com/in/ehrenbolger/